Histological Structure of the Plastinated Kidney Following Deplastination

Plastination is a laboratory process to obtain permanent dry tissue and organ sample. That can be used in the future for investigation educational purposes. This methodology based on dehydration penetration of synthetic substances such as silicon into tissue. In this study, it was aimed deplastinate previously plastinated kidneys order examine them under light microscope. 14 sheep were used, seven samples control plastination-deplastination (p / d) group. Kidneys both p/d groups fixed 10% formalin. The group embedded paraffin following routine processing protocol. However, deplastinated alcohol methylbenzene paraffin. 5 μm thick sections obtained from blocks stained with hematoxylin eosin (H&E), periodic acid-shiff (PAS) then examined Typical histological structures observed Small fragments challenging P/d blocks. Morphological visible some pseudo degenerations wrong staining. study first that demonstrates deplastination partially successful evaluating kidney we believe may have limitations due its wide parenchyma compared literature conclusions. Nevertheless, more studies are required develop optimum protocols.